INTRODUCTION:

Dandruff is a primary beautifying problem that poses very inordinate public healthiness uneasiness both in established and emerging countries.¹ The word dandruff (dandruff, dandriffe) is of Anglo-Saxon derivation, a combination of "tan" meaning "tetter" and "drof "meaning" dirty").² Dandruff is a continuing scalp complaint characterized by scaling, longing and redness of the scalp. It occurs when scalp sheds epidermal cells in large thickets.

The skin of scalp reinstates itself about once a month Usually, scalp sheds dead reformatories in nearly unnoticeable way, but intermittently cell revenue becomes remarkably speedy and dead penitentiaries are shed as visible fragments called dandruff.³A cleanse can be noticeable as a surfactant grounding; (i.e. Surface active material) in a suitable form-liquid, solid, or powder which when used under the circumstances quantified will remove surface grease, dirt and skin debris from the hair, shaft and scalp without heart-rending unfavourably the hair, scalp or the wellbeing of the manipulator. The English term shampoo dates back to 1762, denotation "massage." The term instigates from Anglo-Indian shampoo, in significance from Hindi champoo imerative of champna to rub, press the muscles, massage. Today, around are plenty of shampoos for men and women accessible.

A good shampoo will form ample foam almost promptly notwithstanding of the type of water used or the quantity of soil or fat that needs to be undisturbed from the hair. Nonetheless foam formation is not unimpeded to the cleansing significance, but people expressively always favour a high foam merchandise. Some good shampoos are found to have side effects like drying effect on the hair. It makes hair too dry to be handled or combed with. So proper accommodation of the hair is also an imperative deliberation, some shampoos cause touchiness to the eye and a lasting corneal cloud. These should be avoided. The purposes of shampoo are predicted to be various. A good and satisfactory shampoo should have the following characteristics.⁴ Shampoo may be described as a cosmetic grounding meant for the washing of hair and scalp, packed in a expedient form for use. Its primary function is of emptying the hair of accumulated sebum, scalp debris and residues of hair-grooming arrangements. According to the world health organization, herbal medicines are being used by about 80% of the biosphere inhabitants primarily in the evolving countries for primary health care. This plant-based outmoded me-dicinal system endures to play an necessary role in health care.⁵

Dandruff:

The connotation between dandruff and seborrhea dermatitis has at times been provocative. While most detectives regard seborrhea dermatitis of the scalp as severe dandruff, others believe that dandruff should be used to designate any detaching of the scalp.^6,7A normal scalp has few flakes and vigorous looking, horizontal skin. Dandruff is clinical disorder caused by Malassezia (Pityrosporum) classes is of great decorative concern all over the world. Pityrosporium ovale is strongly suspected to play a role in the appearance of the seborrhea dermatitis. Dandruff is known to be controlled by fungistatic ingredients in Anti-dandruff shampoos. Herbal preparation have growing demand in the world marketplace. The natural medications are more acceptable in market since it’s safe and fewer side effect antidandruff shampoo and nutritional shampoo containing vitamin, amino acids proteins hydrolysate. Presently available behaviour of dandruff include beneficial use of zinc pyrithione, salicylic acid, imidazole offshoots, glycolic acid, steroids, and sulphur and coal tar offshoots. However, these go-betweens show certain boundaries, either due to poor clinical efficacy or due to the. Furthermore compliance issues, these drugs are impotent to prevent repetition. The herbal shampoo powder was framed using natural apparatuses with Acacia concinna (shikakai), Lawsoniainermis (henna), Aloevera (aloe), Ocimum temple (Tulsi), Azadiracta indica (Neem), and Fenugreek (methi). Both are consuming antidandruff action. The synthaticshampoo contain cationic, anionic and non-anionic surfactant mix in this surfactant having good foming charcter but its toxic and caused annoyance of eye. Hard water the surfactants leave a deposit of sodium, calcium and magnesium salts on the hair channel. So these manufactured shampoos are found to consume side effects like drying effect on the hair. These shampoos permission the hair too dry to handle (or) comb, to circumvent these problems, herbal shampoos will be useful.⁸

Defination of Shampoo:

A shampoo may be described as a cosmetic groundwork meant for the washing of hair and scalp, packed in a form expedient for use. Its primary function is of cleansing the hair of gathered sebum, scalp debris and remainders of hair-grooming preparations. The added occupations of shampoo include lubrication, habituation, bodybuilding, prevention of static charge build up, medicine and so on. Finally, the comprehensive shampoo formulation must be therapeutically safe for long-term usage.⁹Hair is an significant part of the overall appeal of the human body. The hair of the head has historically been accompanying with beauty and social difference. Innumerable instances from all the art forms can be cited supporting the special distinction accorded to the coat by people of virtually all times and cultures. Whereas the hair has been trimmed, shaped and even colored meanwhile the most prehistoric times, relatively little emphasis has been placed on the process of cleaning it. Only in this century has a real technology in the cleaning of the hair and scalp been developed. First came the mass distribution of cake soap and sanitary facilities to make corporeal cleanliness and personal hygiene practice. Next came the specialization of branded shampoo products for the hair and scalp, obtainable in multiplicity of types and forms. Now, washing the hair and scalp with shampoo has become a nearly worldwide practice. Shampoos are probably the most widely used hair products today, based on synthetic ingredients as well as herbal components. A shampoo is a groundwork of a surfactant (i.e. surface active material) in a suitable form – liquid, solid or powder – which when used underneath the quantified conditions will eliminate surface grease, dirt, and skin debris from the hair shaft and scalp without unfavourably affecting the user.¹⁰

Composition of Shampoos:

The foodstuffs used to make shampoos are as follows. We comprise key surfactants. e.g. Sodium lauryl sulphate, lauryl sulphate Triethanol. For eg, dialkyl sulphosuccinates, monoalkyl sulphosuccinates, secondary surfactants. E.g., salicylic acid, Benzoic acid, disinfectants and antidandruff agent. Packaging agents e.g. Fatty constituents such as lanolin, and fats. E.g. 4- methyl-7-diethylamino coumarin is a perlescent agent. Requisitions, e.g. EDTA Sodium Salt. Thickeners, e.g. They are alginates. Examples of preservatives are formaldehyde, methyl paraben, propyl paraben. Solubilizing mediators such as aliphatic alcohols, urea and so on.¹¹

The action of shampoo on hair:

The innovative prime meaning of the shampoo is to clean the hair. There are three types of hair soil to be dealt with. These are oilythat soil or sebum, answerable soils, and unsolvable particulate soils. All three types of soil demand being wetted, thus surface tension of the water is reduced by the shampoo surfactant allowing full communication with the soil’s surface. Any answerable soil is then removed in the aqueous medium. A process known as roll-up, i.e, the displacement of the soil by the detergent solution, lremoves oily soil or sebum. Insoluble particulate soils tend to be removed by electrostatic repulsion between the soil and the hair fiber assisted by revulsion between the surfactant molecules adsorbed onto the hair fiber and those softened onto the soil. In the process of soil removal, the cleanser micelles make contact with the lipid surface for a finite time during which they take up an augmentation of lipid. This is conformed to form lipid–detergent co-micelles which detach and float away into the bulk aqueous solution.¹² Hair is an significant part of the overall appeal of the anthropological body. The mane of the head has archeologically been associated with appeal and social distinction. Uncountable instances from all the art forms can be cited supporting the special distinction accorded to the hair by people of virtually all times and cultures. Whereas the hair has been trimmed, shaped and even colored subsequently the most ancient times, moderately little emphasis has been placed on the process of housework it. Only in this century has a real knowledge in the scrubbing of the hair and scalp been developed. First came the mass distribution of cake soap and sanitary conveniences to make bodily sanitation and personal hygiene practice. Next came the concentration of branded shampoo foodstuffs for the hair and scalp, offered in assortment of types and forms. Now, laundry the hair and scalp with shampoo has developed a nearly universal practice. Shampoos are perhaps the most widely used hair foodstuffs today, based on synthetic constituents as well as herbal ingredients.⁸

Anatomy and Physiology of the Hair:

Structure of Hair:

A shock is composed of columns of dead, keratinized cells welded composed. The shaft is a insincere portion of the hair, which projects from the surface of the skin. The shaft of straight hair is rounded in cross section, that of wavy hair is oval and that of wooly hair is lengthened or kidney shaped. The root is the helping of the hair deep into the surface that penetrates into the dermis and occasionally into the subcutaneous layer. The channel and root both consist of three concentric layers-

Medulla:

It is the central part of the shaft and is commonly noticeable in thick hair. It is self-possessed of two or three rows of polyhedral cells comprehending pigment granules and air spaces.

Cortex:

It is located outlying to the medulla and forms the major part of the shaft. It contains of lengthened cells, encompassing pigment particles in dark mane while air in white hair.

Cuticle:

It is the remotest layer of the hair and consists of a single layer of squeaky, flat cells, which are heavily keratinized.

Physiology of the hair:

A hair ascends from the integrated activities of numerous keratinocyte layers in the hair follicle. The expansion of hair is a dynamic, cyclic process in which the duration of growth cycles is synchronized by many hormones and cytokines and depends not only on where the hair is mounting but also on some other factors, such as the personality’s age and stage of expansion, nutritional habits, or conservational alterations like day-length.¹³

Types of Shampoos:

Fig. Anatomy of hair:

1. Powder shampoo

2. Liquid shampoo

3. Lotion shampoo

4. Cream Shampoo

5. Jelly Shampoos

6. Aerosol Shampoo¹⁴

MATERIAL AND METHOD:

The dissimilar parts of the plants were designated for the study consuming haircare belongings. The plants are methi powder, Neem leaf (Azadirachta indica), Shikakai fruit (Acacia concinna), Aloe leaf (Aloe barbadensis), Henna Leaf (Lawsonia inermis), Brahmi root (Centella asiatica), Ritha fruit (Sapindus mukorossi), Amla fruit (Embelica officinalis), Nagarmotha (Cyperus rotundus) and Tulsi. The precipitous of methi, Amla fruit, Neem leaf, Shikakai fruit, Aloe leaf, Henna Leaf, Ritha fruit were collected from the local market. The raw ingredients collected were given with their corresponding biological source and uses in (table no.1) ingredients in the haircare; even they are accountable to provide the nutrition to the body. Herbs have long been accompanying with haircare and are often ingredients of conditioners, shampoos and rinses. The selection of active constituents for haircare precipitate is often based on the ability of the component to prevent damage to the skin as well as to improve the excellence of the skin by way of cleansing, wholesome and defensive the skin. In the paper, we reported the expansion and assessment of herbal haircare powder.¹⁵

Colleaction of Plant:

Additional parts of Hibiscus rosea sinesis (hibiscus leaves and flowers), Azadirachta indica (neem leaves), Lawsonia inermis (heena leaves) and aloe leaves were collected from vegetal garden and washed under consecutively water to remove pollutants while Sapindus mukorossi(reetha), Acacia concinna (shikkaai), Phyllanthus emblica (amla) and Trigonella foencum (methi seeds)were composed in dried method from market and then authentic from department of botany, Vinodini PG college, Shekhawati university, Rajasthan. All the avocado fresh constituents were dried in shade, converted into coarse precipitates and sieved. The raw resources were given with their biological source and uses correspondingly.¹⁶

Evaluation of Shampoo Formulation:

A. Development formulation was appraised for their clarity, color and odour. All evaluations where reported and deliberated.

B. Determination of PH:

Developed preparation was diluted using concentrated water to pre-pare a example with 10% concentration. The prepared sample was chequered for ph consuming a digital ph meter at room temperature 30±2°C.

C. Mesurment of Viscosity:

The thickness of the shampoo was strong-minded by using Brookfield Viscometer LVDV Prime-I. The viscosity of shampoo was unhurried at room temperature i.e. 30±2°C with fluctuating rpm and torque.

D. Foaming Ability and Foaming Stability:

Chamber shake method with slight alteration was used for deter-mining effervescing ability. 50ml of the 1% shampoo solution was put into a 250ml progressed measuring cylinder and covered with hand. Measuring cylinder was shaken for 1 minute. The total volume of the foam substances after 1 tiny shaking was chronicled. The procedure was constant for 5 minutes.

E. In Vitro Anti-Danddruff Activitie:

Candida albicans were working for testing antifungal movement using the cup-plate method. The culture was preserved on sabouraud’s agar slants. 20ml of liquefied sabouraud’s agar medium was immunize-

End with 0.2ml of 72 hour old postponement of Candida albicans and poured in to a petri dish. The cups (10mm diameter) were perforated in the petri dishes and jam-packed with 0.05ml of a solution of the sample liquefied in DMSO The plates were kept for diffusion at 40C for one hour and reared at 30⁰C for 48 hours. After the accomplishment of incubation period, the zone of embarrassment in millimeter were measured.¹⁷

G. Determination of Percentage Solid Contain:

Four grams of the organized shampoo were placed in a clean dry vanishing dish. The heaviness of the dish and shampoo was determined. The liquid helping of the shampoo was vanished by placing it on a hot plate. After complete drying the weight of the shampoo solid contents was single-minded.

H. Stability of Study:

Twenty-four sets of 60-gram shampoo (1% olive leaves extract) were stored at different temperatures ambient malaise, for 6 months. After each month, their steadiness was checked apropos Oleuropein content, entrance, ph, Color and Viscosity

I. Determination of Oleuroepin in Shampoos by Hplc:

For willpower of Oleuropein in cleanses, the reversed phase HPLC method was used with silica-based C18 bonded phase support (5µm, 150 × 4.6mm inner diameter) and a mobile phase consisting of acetonitrile/ phosphate buffer ph 3.0 (20:80 v/v) at a flow rate of 1.0 ml/minute and UV uncovering at 280nm.

J. Evaluation of Herbal Shampoos:

To estimate the prepared constructions, quality control tests including organoleptic and physiochemical classification such as ph, solid innards and viscosity were completed. To ensure the quality of the products, explicit tests for shampoo preparations including foam volume and foam firmness in addition to stability study were also accepted out.¹⁸

Preliminary Stability Test:

Stability and adequacy of organoleptic properties (odor and color) of constructions during storage indicate chemical and physical stability of the organised shampoos formulations. This study was directed on a candidate formulary Samples were positioned in dark glass tubes and stored in a chamber at 45°C and 75% relative humidity. Their entrance and corporeal stability was inspected for a period of 3 months at interval of one month 13, 14. To assess their motor-powered stability, each sample was centrifuged at 2400rpm for 3 minutes and then its structural stability was examined.

Statistics Analysis:

The results were prearranged as mean±S.D. (n=5). One-way analysis of modification (ANOVA) judgement test was used to compare characteristics of different preparations with the marketable product. A p value of 0.05 was painstaking to be significant.

Cleaning Action:

Scrubbing action was tested on wool yarn in blubber. Though cleaning or soil/sebum removal is the primary aim of a shampoo, experimental detergency estimation has been difficult to normalise, as there is no real agreement on a standard soil, a reproducible soiling process or the amount of soil a shampoo should ideally remove. As seen from the results, there is a significant difference in the amount of sebum removed by the diverse shampoos. The results of detergency studies showed that the final formulation has significantly similar detergency ability, when compared with the promoted formulations and it was found in between 18-33%.¹⁹

Physiochemical Evaluation:

1. Solubility:

Two ml of the shampoo is added to 100ml of water. Thesolution formed was well surprised and then heated to increase solubility. After 10 mins of heating the key was cooled down and then the measure of remainder was noted.

2. Washability:

Designs were functional on the skin and then washed off to perceive the ease and extent of washing of the inventions by water.

3. pH:

The pH is considered by pen pH Meter. The pH was relaxed of 10% Shampoo solution in distilled water. 5 identifications were taken and the mean of it was painstaking as the pH of shampoo explanation.

4. Skin and Eye Errition Test:

The skin frustration test was done as per mentioned procedure. The explanations showed no Redding or swelling of the covering. Also there were no itching sensations. The test was done at about 10 undertakes which were found totally healthy before and after the tests. Steadiness study of the shampoo was done by ownership the shampoo in a hairtight ampule at cool and dry place for 3 days. The shampoo was experimental for changes in color, touch, etc. at intervals of 12 hrs. 24 hrs. 48 hrs. 72 hours Nature of hair after washing:-Nature of hairs after laundry by the shampoo can be noted by the rejoinders of the candy striper taken to test the shampoo on their hairs for about 3 hair shampoos.²⁰

REFERENCES:

1. JR Krishnamoorthy, African Journal of Biotechnology Vol. 5 (10), pp. 960-962, 16 May 2006.

2. Ranganathan and Mukhopadhyay, CME Article2010, Volume-55, page:130-134evaluationme

3. Loden and Wessman, International Journal of Cosmetic Science 2001, Volume22, Issue4

4. B.M. Mithal, R.N. Saha, A hand book of cosmetics, first edition, 2000. V P Kapoor, (2005), Herbal cosmetics for skin and hair care, IntJ. Nat. Prod. Plant Re-sour, Vol 4(4), pp no: 306-314

5. A Bulmer, G Bulmer, The antifungal action of dandruff shampoos, Mycopathologia 1999;147: 63-65.

6. CJ Pierard Franchimont, JF Hermanns, H Degreef, GE Pierard, From axioms to new insights into dandruff, Dermatology 2000;200: 93-98 .

7. Evans, WC “Pharmacognosy”, 16th Ed., Harcourt Brace and Company, New York 14 Ltd.1997;16:28.

8. William, D.F. and Schmitt, W.H. (1992). Chemistry and Technology of Cosmetic and Toiletries Industry. Blackie Academic and Professional, New York, pp. 356-408.

9. Khandelwal, KR “Practical Pharmacognosy Techniques & Experiment”, Nirali Prakashan. 2008;19:102,106.

10. Jaya Preeti P. Padmini K., Srikanth J, Lohita M, Swetha K Vengal rao p., a review on H Herbal Shampoo and its Evaluation, Asian J. Pharm. Ana. 3(4): 2013; 153-156 3.

11. Sharma, P.P. (1999). Shampoos. In: Cosmetics- Formulation, Manufacturing and Quality Control. 4th ed. Vandana Publications Pvt. Ltd. Delhi, pp. 323-348.

12. Wolfram LJ. Human hair: a Unique Physicochemical Composite. J Am Acad Dermatol., 2003; 48: S106– S114.

13. Ali Saad, Rasool Kahim. Formulation and evaluation of herbal shampoo from Ziziphus spina leaves extract. International Journal of Research in Ayurveda & Pharmacy.

14. Sachin Gholve, Sachin Nandarge, Sunil Hindole, Ompraksh Busnure, Pratab Bhosale and Sanjay Thonte. Formulation and evaluation of Polyherbal, Anti-danddruf powder shampoo. World Journal of Pharmacutical Research, Volume no. 04, Issue -10

15. Jyoti Ghalawat, Devender Sharma, Ganjendra Thakur, Jithendra Chobdar, Vivek Sharma. Formulation and evaluation of Polyherbal liquid shampoos. European Journal of Biochemical and Pharmaceutical Science, Volume no. 6,

16. Sarath Chandran, Vipin K. V, Anil Shirwarkar. Development and evaluation of anti-dandruff shampoo bases on natural source. Journal of Pharmacy and Pharmcotheraputic.

17. Hiba Yatim, Michael Hanania and Nida Mosleh. Formulation and evaluation of herbal shampoo containing olive leave extract. International Journal of Development Research, volume no. 08, Issue on 10.

18. Ali Saad, Rasool Kahim. Formulation and evaluation of herbal shampoo from Ziziphus spina leaves extract. International Journal of Research in Ayurveda & Pharmacy.

19. Gaurve Lodha. Formulation and evaluation of Polyherbal shampoos to promote hairgrowth and anti-dandruff action. Journal of drug Deliver and Therapeutic.

Received on 17.02.2022 Modified on 14.04.2022

Asian J. Pharm. Res. 2022; 12(3):212-216.

DOI: 10.52711/2231-5691.2022.00035